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Introduction Bioprinting has emerged as a powerful tool for the fabrication of highly hierarchical, organized tissue equivalents, comprising cells, bioactive molecules and biomaterials in a spatially defined arrangement. Based on these studies, future research should consider mc as an auxiliary material for bioinks and biofabricated constructs with high shape fidelity. Moreover, a brief overview about first bioprinted functional tissue equivalents is given, which have been fabricated by using mc. This minireview highlights the multiple applications of mc for biofabrication: it was successfully used as sacrificial ink to enable 3D shaping of cell sheets or biomaterial inks as well as as internal stabilizing component of various bioinks. Recent studies showed the great potential of the polysaccharide methylcellulose (mc) as supportive biomaterial that can be utilized in various ways to enable biofabrication and especially extrusion-based bioprinting of bioinks.

Concepts enabling printing of cell-laden constructs with high shape fidelity were developed only rarely. First bioprinting studies introduced bioinks allowing either good cell viability or good shape fidelity. A prominent technique allowing biofabrication of tissue equivalents is extrusion-based 3D (bio)printing, also called 3D (bio)plotting or robocasting, which comprises cells embedded in the biomaterial (bioink) during the fabrication process. 2012: DJ Harsh & Guè Pequeno – Fast Life Vol.With the aid of biofabrication, cells can be spatially arranged in three dimensions, which offers the opportunity to guide tissue maturation in a better way compared to traditional tissue engineering approaches.2009: DJ Harsh & Guè Pequeno – Fast Life Vol.2006: DJ Harsh & Guè Pequeno – Fast Life Vol.2005: Marracash & Dogo Gang – Roccia Music Vol.2005: Guè Pequeno & Deleterio – Hashishinz Sound Vol.2005: Don Joe & Grand Agent – Regular (EP).Ĭlub Dogo stopped producing music together in 2014, although none of the three members ever stated the group was officially disbanded.

In 2005, Club Dogo formed the crew named Dogo Gang, including other MCs and DJs from Milan that gradually joined the collective. In 2001, after their first and only CD (3 MC's al Cubo), the group was disbanded due to quarrels between Jake and Dargen. Originally the group name was "Sacre Scuole" and its members were the two aforementioned rappers and Jake La Furia, whose stage name was originally Fame. The group was born in 1999 from the friendship between rappers Gué Pequeno (whose stage name was Lucky Luciano) and Dargen D'Amico.

For more guidance, see Wikipedia:Translation.Ĭlub Dogo was an Italian rap group from Milan ( Lombardy) that consists of Gué Pequeno and Jake La Furia, and beatmaker Don Joe.

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